Comments (4)
Thank you for reporting! I will try to fix all this asap (hopefully over the weekend).
I will implement:
- A parameter
--prefix
to set custom outfile name - A parameter
--index
for specifying index location. - Removal of temporary files
- Update documentation
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So I implemented the above points 1-4. The new version (v0.0.3.2) is available to install with conda/pip.
For example
./uLTRA pipeline test/SIRV_genes.fasta \
/path/to/test/SIRV_genes_C_170612a.gtf \
test/reads.fa outfolder \
--prefix sample_X \
--index index
Will create and use the index located in folder index/
, and the final aligned file will be created as outfolder/sample_X.sam
. Temporary files are also deleted. If they are wished to be kept, this could be specified with option --keep_temporary_files
.
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Looks great!
from ultra.
Some small notes:
for ultra index
- the difference between "outdir" (Path to output folder) and "index" (Path to where index files will be written to [default is the outfolder path].) parameters is unclear, as this step should only prepare the index files, right?
- gtf is explained as "Path to gtf or gtf file with gene models." Should this be gff3 file?
- --disable_infer "Makes splice creation step much faster. Thes parameter can be set if gene and
transcript name fields are provided in gtf file." Isn't this always the case? (and typo "Thes")
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Related Issues (20)
- ultra installation and run error HOT 7
- CIGAR string starts/ends with N HOT 7
- Mapping with uLTRA without GTF? HOT 3
- Cigar is None HOT 2
- BUG -4294967296 HOT 8
- Controlling (high) uLTRA RAM usage HOT 1
- a bug of `--alignment_threshold` HOT 1
- Out of bound reads HOT 13
- Genomes FASTA/GTF files needed to run the evaluation HOT 4
- Can not access local variable 'read_mems_tmp' when using --use_NAM_seeds HOT 2
- Error: invalid feature coordinates (end<start!) at line: HOT 1
- Bug with uLTRA align : TypeError: bad argument type for built-in operation HOT 3
- UnboundLocalError: local variable 'i' referenced before assignment HOT 4
- Can I use ultra to align est to references HOT 1
- error when aligning direct RNA data during revcomp script HOT 3
- KeyError when running test pipeline HOT 3
- How to control minimap 2 parameters during uLTRA alignment HOT 6
- uLTRA + SQANTI3 HOT 2
- Non-absolute paths don't resolve HOT 5
- Python bindings? HOT 1
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