DM to provide WH with flow data providing gDNA concentration for batch to analyze primer competition.

test issue

Need to generate spike dilution data for analysis

Vary gDNA concentration and use constant spike amount

1. Control Experiment
   • Samples containing:
     • Five replicates of five different concentrations of gDNA (100ng, 200ng, 300ng, 400ng, 500ng)
     • Constant spike concentration of (TO DO: what is this value?)
   • Sample types:
     • Transgenic mouse 1
     • Transgenic mouse 2
     • 50-50 mix of Transgenic mice 1 and 2
     • WT mouse 1
   • 100 total samples

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WH to analyze the data DM provides in #6

Plot and perform linear regression on [gDNA] and total spike counts

• Ho: Total spike counts do not differ depending on the [gDNA] in the sample
• Ha: Total spike counts do differ depending on the [gDNA] in the sample

Repeat above, for individual spike counts

• Ho: Individual spike counts do not differ depending on the [gDNA] in the sample
• Ha: Individual spike counts do differ depending on the [gDNA] in the sample

Input

results from #4

Output

Plot and perform linear regression on [gDNA] and total spike counts

• Ho: Total spike counts do not differ depending on the [gDNA] in the sample
• Ha: Total spike counts do differ depending on the [gDNA] in the sample

Repeat above, for individual spike counts

• Ho: Individual spike counts do not differ depending on the [gDNA] in the sample
• Ha: Individual spike counts do differ depending on the [gDNA] in the sample

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