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LSTrAP-denovo is an easy-to-use pipeline to assemble and annotate transcripts for species without genome / genomic annotations using publicly available RNA-seq data.

License: MIT License

Python 100.00%
bioinformatics clustering-algorithm gene-expression pfam-annotation rna-seq rna-seq-pipeline transcriptomics-assembly unsupervised-machine-learning

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lstrap-denovo's Issues

SOAPdenovo make failed with GCCcore-11.3.0

I had trouble installing SOAPdenovo through the setup.py script. It failed to build (see error below). It would be great to have this pipeline available through a Conda package/Docker/Singularity for portability.

/sw/auto/rocky8.6/epyc3/software/binutils/2.38-GCCcore-11.3.0/bin/ld: splitReps.o:(.bss+0x0): multiple definition of `kmerZero'; arc.o:(.bss+0x0): first defined here
/sw/auto/rocky8.6/epyc3/software/binutils/2.38-GCCcore-11.3.0/bin/ld: splitReps.o:(.bss+0x20): multiple definition of `b_ban'; arc.o:(.bss+0x20): first defined here
/sw/auto/rocky8.6/epyc3/software/binutils/2.38-GCCcore-11.3.0/bin/ld: cannot find -lm: No such file or directory
/sw/auto/rocky8.6/epyc3/software/binutils/2.38-GCCcore-11.3.0/bin/ld: cannot find -lrt: No such file or directory
/sw/auto/rocky8.6/epyc3/software/binutils/2.38-GCCcore-11.3.0/bin/ld: cannot find -lpthread: No such file or directory
/sw/auto/rocky8.6/epyc3/software/binutils/2.38-GCCcore-11.3.0/bin/ld: cannot find -lc: No such file or directory
collect2: error: ld returned 1 exit status
make: *** [Makefile:76: SOAPdenovo] Error 1

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