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View Code? Open in Web Editor NEWpreliminary assessment of data from IA macro
preliminary assessment of data from IA macro
Spline Measure is randomly quitting when it encounters a AddFoci.
In splineMeasure, the sc was selected, and name tested (this might not be nessecary), when the SC was a SC piece from Cleave function, the roiManager name was changed to 'NN cleaved piece'. It is missing the 'SC' that identifies all other SC pieces.
The name can be manually changed in Roi manger to pass through the regex with SC.
Change the macro code to rename cleaved pieces with SC.
I first noticed this in a quite dim image, different SCs disappeared at different times.
Write in an option in the editing section to mark the roi name to mark bad blobjects so that they can be disregarded.
Find a way to rewrite the name with the blobject number. This would require re-writing all blobject names and updating the roi manager. This might not be time effective.
Ideas for making the UI easier for approving and editing blobjects for producing high quality results.
-add obj_class text mask to image before approval
-mouseover text
-new zoomed in window with obj_class text
estimate the number of mice needed for quantification to get good biological estimates
50% of bivalents have what appears to be a centromere orientation error.
tried in G males image and female image. ~50% of position measures are shitty.
This could be due to the print measure instead of the foci macro. I'm not sure how to verify the set aspects / properties.
If the SC doesn't touch the centromere -- length and position measures will be underestimated.
Think of a way to extend SC, perhaps with a spline. (wait for user, point and click spline then add)?
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