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denoising-fluorescence's Issues

Some problems occurred when running in Google Colab

Hi!
Very nice work!
But when I run train_dncnn.py and benchmark.py in Google Colab, it reminds TypeError:

///
Traceback (most recent call last):
File "train_dncnn.py", line 133, in
patch_size=args.imsize, test_fov=19)
File "/content/denoising-fluorescence/denoising/utils/data_loader.py", line 412, in load_denoising
target_transform=target_transform, loader=pil_loader)
File "/content/denoising-fluorescence/denoising/utils/data_loader.py", line 137, in init
self.samples = self._gather_files()
File "/content/denoising-fluorescence/denoising/utils/data_loader.py", line 167, in _gather_files
if is_image_file(fname):
File "/content/denoising-fluorescence/denoising/utils/data_loader.py", line 28, in is_image_file
return has_file_allowed_extension(filename, IMG_EXTENSIONS)
File "/usr/local/lib/python3.6/dist-packages/torchvision/datasets/folder.py", line 20, in has_file_allowed_extension
return filename.lower().endswith(extensions)
TypeError: endswith first arg must be str or a tuple of str, not list
///

Do you know how to deal with it? Thank you so much.

Problem with the download of the datasets

xxxx> bash download_dataset.sh confocal
download_dataset.sh: line 33: [: missing `]'
Downloading dataset Confocal_BPAE_B...
--2021-09-13 14:03:54-- https://docs.google.com/uc?export=download&confirm=&id=1juaumcGn5QlFRXRQyrqfbZBhF7oX__iW
Resolving docs.google.com (docs.google.com)... 142.250.203.110, 2a00:1450:400a:801::200e
Connecting to docs.google.com (docs.google.com)|142.250.203.110|:443... connected.
HTTP request sent, awaiting response... 403 Forbidden
2021-09-13 14:04:00 ERROR 403: Forbidden.

Extracting files from ./dataset/Confocal_BPAE_B.tar...
tar: This does not look like a tar archive
tar: Exiting with failure status due to previous errors
Seems the tar file is wrong, download again...

How to extend the program to 16-bit tif pictures?

Hello, I'm sorry to bother you.

But as far as I know, most images in the field of biomedicine are 16-bit tif images.

I tried some 16-bit images in the program, but because the PIL package cannot read 16-bit tif images, the program cannot run. After that, I converted the tif file into a 16-bit png format and sent it to test_example.py for testing. At this time, the image can be read, but the results are blank images. Then I converted the image to 8-bit png format, this time the program successfully completed the denoising task.
n2n_noise1_1_test19_idx0_denoised
But 8-bit is missing a lot of information compared to 16-bit. So I would like to ask how to make this program run smoothly on 16-bit image set? And how to call the program when inputting 1024 * 1024 images?

thank you very much!

Why there is a big difference between the brightness of concocal BPAE obtained by network training and ground truth

Hello, I'm sorry to bother you.

I retrained the DnCNN and Noise2Noise networks, tested the model, and got the denoising image in the test mix data set. I combined the images of three channels of focal BPAE into color images, but found that the resulting images are very different from the ground truth (color).

Confocal_BPAE_3_gt
n2n-Confocal_BPAE_3

The gray images obtained from network training are all three-channel, while the gray images in the ground truth are single channel. I guess it may be related to this, but no solution has been found. How to deal with this?

could this dataset applied for x-ray deep denoising?

Mentioned in the article: "This work is dedicated for fluorescence microscopy denoising where the images are corrupted by Poisson-Gaussian noise; in particular,Poisson noise, or shot noise, is the dominant noise source"

Due to the lack of dataset in x ray field(not in medical x-ray field),I want use this dataset for training. So could this dataset applied for x-ray deep denoising?the x-ray images are mainly corrupted by poisson noise.

In addition ,the dataset URL cannot be opened in mainland China,

hope for your reply!

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