TypeError: '>=' not supported between instances of 'NoneType' and 'float' about ribotish HOT 3 CLOSED

TIS group has None value. Does your GTF have only part of genes? If so, try to use full annotation.
Another warning problem is that the para file was not loaded with --tispara. Try the default name of LTM.bam.para.py. What's your -b bam data?

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Does your GTF have only part of genes?

The GTF has all of the genes and includes exons and CDS. It does not have Gene or Transcript entries.
When I did the original alignment I used a different GTF in STAR. Could this be problematic?

Another warning problem is that the para file was not loaded with --tispara.

Ah yes I passed the wrong file name. Thanks!

What's your -b bam data

I mapped multiple fastqs from cycloheximide treated samples. Then I merged with samtools merge to get "merge.bam" which I use as input

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GTF with only exons and CDSs should be OK. You can use -a option to provide gene annotation other than prediction objects.
Why you used a different GTF?
You can try to use smaller number of groups, for example option --nparts 5, and the None type may disappear.

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