Comments (1)
Hi,
The error is a bug. I'll fix it later.
By the way,
The TISDIFF module is only for quantitative TI-Seq (QTI). Your data looks like harr type TI-Seq, which is not quantitative. The differential results may be not very accurate.
The offset parameter files should be like Sample_6/sample1_sort.bam.para.py, which are generated using ribotish quality. The parameter files record estimated P-site offset values. You need to check the quality pdf figures generated by quality module. Don't forget to use -t when running TI-Seq quality.
Best,
Peng
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